Ethnobotany of dye plants in Southern Italy, Mediterranean Basin: floristic catalog and two centuries of analysis of traditional botanical knowledge heritage
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Ethnobotany of dye plants in Southern Italy, Mediterranean Basin: floristic catalog and two centuries of analysis of traditional botanical knowledge heritage
Background: Since ancient times, humans have learned to use the plant to obtain natural dyes, but this traditional botanical knowledge (TBK) is eroding. In the late 1800s, during, and early, there is an increase in research related to the coloring species, and this allows the industrial and economic development within the context of rural southern Italy. Today, the dye is mainly derived from synthetic products, and this leads to human health risks associated with pollution.
Methods: From the literature, three catalogs of dyeing species (plants, algae, fungi, and algae) used in the Mediterranean Basin and especially in southern Italy has been created. The percentage of parts used and the color extracted from the species have been recorded and analyzed. The plant species that exist in the catalog has been verified in the region of Southern Italy, and the data that has been registered. A survey conducted ethnobotany, in the region of Southern Italy, to verify the level of erosion of traditional botanical knowledge, associated with dyeing ethnobotany, from time to time.
Results: A total of 524 species recorded in plants, algae, fungi, and algae, and related parts used and extracted pigments. Most use concern stems and leaves, and the most frequent color is yellow. Of survey operations in the field, 283 species of plants have been verified. This represents 64.31% of the species reported in the resulting dye plant flora. The result, of ethnobotany survey showed that only 8.6% of the ICC remain in the collective memory.
Conclusion: This catalog is one of the largest in the sector and is the basis for studies related to the eco-sustainable economic recovery that will enable the development of marginal region is present throughout southern Italy.
Ethnobotany of dye plants in Southern Italy, Mediterranean Basin: floristic catalog and two centuries of analysis of traditional botanical knowledge heritage
Ethnobotany, Ethnopharmacology, phytochemical, biological activity and toxicity of Pistacia chinensis subsp. integerrima: A comprehensive review
Pistacia chinensis subsp. integerrima (J. L. Stewart ex Brandis) Rech. F. is a valuable medicinal plants used in south Asian communities for the treatment of asthma, diarrhea, diabetes, liver disease, fever, pain and inflammation. This review critically evaluate the information provided in this integerrima P. ethnobotany, Ethnopharmacology, phytochemical, pharmacological and toxicology.
Electronic databases such as Google Scholar, PubMed, Springer Link, and so on, books and thesis which is used to find relevant information about P. integerrima using keywords such as “Pistacia integerrima,” “P. integerrima,” “Ethnopharmacology,” “Phytochemistry, ” ‘Traditional use’. A number of in vitro and in vivo pharmacological activity has been reported; However, the activity of the most promising and interesting observation is its role in Alzheimer’s, diabetes, seizures, cancer, asthma, diabetes, diarrhea and as immunomodulatory, analgesic and anti-inflammatory.
Moreover, given the sour Pistagremic anti-Alzheimer’s activity based on hitherto unknown mechanism through interference with the amyloidogenic pathway. Most of the pharmacological activity associated with the traditional use. Various compounds have been reported from P. integerrima including triterpene extract, essential oils, flavonoids, fatty acids, phenolics, phytosterols, tannins and oligosaccharides and unknown triterpene and flavonoids.
SCRIPTUM High Precise - One-Step RT-PCR, 100 reactions
Description: SCRIPTUM High Preciseis aone-step kitforreverse transcription (RT) of specific RNA templates followed by polymerase chain reaction (PCR) .SCRIPTUM High Precise is ideal for highly sensitive reverse transcription and high fidelity PCRs with high amplification speed. The kit contains a genetically modified reverse transcriptase with improved thermal stability. The SCRIPTUM reverse transcriptase is highly specific and allows high cDNA yields and improved efficiency for complex, long cDNA fragments. A genetically engineeredpolymerase with proof-reading activityoffers 50x higher accuracy and increased processivity, with 2x shorter elongation times, compared to Taq polymerase. The first-class SCRIPTUM High Precise Enzyme Mix in combination with the optimised SCRIPTUM High Precise Reaction Buffer (with high-purity dNTPs) guarantees excellent amplification results.SCRIPTUM High Precise contains all reagents required for RT-PCR (except RNA template and primer) . The reagents are mixed in a single reaction tube and the reaction can be performed sequentially without having to open the tube. This guaranteesquick and easy performance with a minimum of pipetting steps and a reduced risk of contamination.PropertiesOne-Step RT-PCR KitSuitable for specific RNA templatesContains all necessary reagents except RNA template and primerGood to knowThe principle of RT-PCR consists of the synthesis of cDNA by reverse transcription (RT) of an RNA template and subsequent amplification of the cDNA by polymerase chain reaction (PCR) .The first step of RT-PCR consists of converting RNA into complementary DNA (cDNA) . This is done by the enzyme reverse transcriptase, which uses the RNA as a template to synthesise the cDNA. Once the cDNA has been synthesised, PCR is carried out to amplify the cDNA. Specific primers are used that bind to the cDNA. New DNA strands are synthesised with the help of a DNA polymerase. RT-PCR is used in gene expression analysis, in the diagnosis of infectious diseases and in basic research.Different RT-PCR types:Quantitative RT-PCR (qRT-PCR) : This method enables the quantitative determination of the amount of RNA in a sample. It uses fluorescent dyes or probes to monitor amplification in real time.Semi-quantitative RT-PCR: This method allows an approximate quantification of the RNA by analysing the amplification in different cycles, but not in real time.Multiplex RT-PCR: Several target genes are amplified in a single reaction, which saves time and material.
Description: Globe Scientific's reaction vessel is produced from polypropylene (PP) and is designed for use with the Abbott® Architect® Series Analyzers. The reaction vessels are packaged 2000/bag.
Description: DescriptionFor research use only. Not to be used for diagnostic purposes.Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using Real-Time quantitative PCR (RT-qPCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of pneumonia outbreak. This Coronavirus detection kit allows efficient cDNA synthesis and qPCR in a single tube. This probe based one step RTqPCR 2x master mix contains Reverse Transcriptase, Taq DNA polymerase, RNase inhibitor, MgCl2, dNTPs, stabilizers and low ROX reference dye with proprietary buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/ probe sets. This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product Includes:RT-qPCR 2x master mixCDC recommended primer/probe setsCOVID-19 positive control (PTC)
Description: TDO Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for TDO-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS TDO Inhibitor Screening Assay Kit (BPS Cat. #72023) and/or with purified, active TDO, His-tag (BPS Cat. #71195) in combination with 1x TDO Assay Buffer (BPS Cat. #73006).
Description: IDO1 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO1-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO1 Inhibitor Screening Assay Kit (BPS Cat. #72021) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002).
Description: IDO2 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO2-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO2 Inhibitor Screening Assay Kit (BPS Cat. #72022) and/or with purified, active IDO2, His-tag (BPS Cat. #71194) in combination with 1x IDO2 Assay Buffer (BPS Cat. #73004).
HCV Real-TM Quant
Real Time PCR Test for quantitative detection of HCV (25 μl Reaction Mix)
Description: Blood Direct 2x PCR Master Mix enables fast and easy amplification of target DNA from whole blood samples. The master mix is optimized for DNA amplification directly from whole blood samples and does not require prior DNA purification or lysis of the blood sample. The ready-to-use master mix is 2x concentrated and contains all reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Properties:Ready-to-use- Transfer blood sample (1-3 µl) directly into the master mix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform: No complex, time-consuming lysis, DNA isolation and washing stepsHigh sensitivity- Excellent for genes that are frequently examined in the same way in routine screeningSuitable for genomic DNA from blood samples
Description: Blood Direct 2x PCR Master Mix enables fast and easy amplification of target DNA from whole blood samples. The master mix is optimized for DNA amplification directly from whole blood samples and does not require prior DNA purification or lysis of the blood sample. The ready-to-use master mix is 2x concentrated and contains all reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Properties:Ready-to-use- Transfer blood sample (1-3 µl) directly into the master mix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform: No complex, time-consuming lysis, DNA isolation and washing stepsHigh sensitivity- Excellent for genes that are frequently examined in the same way in routine screeningSuitable for genomic DNA from blood samples
Description: Plant direct 2x PCR Mastermixis optimized for the amplification of target DNAdirectly from plant cells or seedsand does not require prior DNA purification. The ready-to-use Mastermix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Plant direct 2x PCR Mastermix is supplied in combinationwith an optimized extraction buffer, which enables rapid lysis (approx. 5-10 min) of your plant parts. Plant direct 2x PCR Mastermix enables quick and easy PCR preparation from plant cells, plant tissue and seeds.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: Tissue Direct 2x PCR master mixis optimized for the amplification of target DNAdirectly from human or animal tissueand does not require prior DNA purification. The ready-to-use master mix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Tissue direct 2x PCR master mix is delivered in combinationwith an optimized extraction buffer, which enables a fast lysis (approx. 5-10 min) of your tissue. Tissue direct 2x PCR master mix enables fast and easy PCR preparation from human and animal tissue as well as cell lysates.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: Tissue Direct 2x PCR master mixis optimized for the amplification of target DNAdirectly from human or animal tissueand does not require prior DNA purification. The ready-to-use master mix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Tissue direct 2x PCR master mix is delivered in combinationwith an optimized extraction buffer, which enables a fast lysis (approx. 5-10 min) of your tissue. Tissue direct 2x PCR master mix enables fast and easy PCR preparation from human and animal tissue as well as cell lysates.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: Plant direct 2x PCR Mastermixis optimized for the amplification of target DNAdirectly from plant cells or seedsand does not require prior DNA purification. The ready-to-use Mastermix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Plant direct 2x PCR Mastermix is supplied in combinationwith an optimized extraction buffer, which enables rapid lysis (approx. 5-10 min) of your plant parts. Plant direct 2x PCR Mastermix enables quick and easy PCR preparation from plant cells, plant tissue and seeds.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Pistagremic acid, a triterpene novel, is due to most activities. in vivo toxicology studies in animals suggest the toxic dose of 1500 mg kg-1, for its methanol extract. All reported pharmacological activities carried out in vitro and gaps in the research, namely, preclinical and clinical investigations there. Outstanding activities as an agent antiglycating is the most promising and unique as far as activity and requires further evaluation. In-depth research and clinical trials on human subjects to investigate P. integerrima pharmacological activities, clinical efficacy and safety is an important next step.
