Ethnobotany, phytochemistry and pharmacological significance of the genus Bulbine (Asphodelaceae)
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Ethnobotany, phytochemistry and pharmacological significance of the genus Bulbine (Asphodelaceae)
Ethnopharmacological relevance: The genus Bulbine (Asphodelaceae) is spread in South Africa and Australia and has been traditionally used for a variety of medicinal applications such as treating skin diseases, burns, diarrhea, and sexually transmitted diseases.
The purpose of this review: The aim is to present a critical review of important species of the genus Bulbine ethnomedicinally with a comprehensive overview of the chemical content and biological activity.
Materials and methods: This article is an overview of published literature on the genus Bulbine in six decades with regard to the composition of phytochemical and pharmacological potential of each with the help of the data obtained from the search engine Google Scholar search string is done using key words to get relevant publications of scientific databases including ACS Journals, PubMed, Science Direct, SciELO, Sci Finder, Springer, Tailor & Francis, the Plant List database, web of Science and Wiley.
Results: The literature survey revealed that only 12 species in the genus Bulbine has been reported to be used by traditional scientific record Anthraquinones ethnomedicinal use emerged as the most abundant phytochemicals in the genus. metabolite isolated / detected more including isofuranonaphthoquinones, flavonoids and triterpenoids. Promising pharmacological activity has been reported by members of the genus by antiplasmodial, antitrypanosomal, antiviral, antioxidant, anticancer, anti-inflammatory activity and anti-microbial, a powerful wound healing properties and to improve reproduction.
Conclusion: This review shows the traditional use of this genus and preventive and curative properties in the management of diseases listed support of bioassay test compounds and extracts. State-of-the-art analytical techniques required for the characterization and quantification of compounds in the genus. The efficacy of the therapeutic potential of the species Bulbine requires further confirmation with pre-clinical studies and clinical.
Ethnobotany, phytochemistry and pharmacological significance of the genus Bulbine (Asphodelaceae)
Ethnobotany and Antimicrobial Peptides From Plants Family Solanaceae: An Update and Future Prospects
Solanaceae is an important plant families that have played an important role in traditional medicine and human nutrition. Rich in bioactive metabolites Solanaceae members and has been used by different tribes around the world for centuries. antimicrobial peptides (AMP) of the plant have attracted great interest in recent years and raised new hope for developing new antimicrobial agents to face the challenges of antibiotic resistance.
This review aims to summarize the AMP reported from plants of the Solanaceae with the possible molecular mechanisms of action and correlate them with the traditional use of antimicrobial action of peptides reported. A systematic literature review conducted by using a different database until August 2019 based on inclusion and exclusion criteria. According to the literature, various AMP including defensins, protease inhibitors, lectins, thionin-like peptides, vicilin-like peptides, and snaking isolated from plants of the Solanaceae and involved in their defense mechanism.
Description: Globe Scientific's reaction vessel is produced from polypropylene (PP) and is designed for use with the Abbott® Architect® Series Analyzers. The reaction vessels are packaged 2000/bag.
Description: DescriptionFor research use only. Not to be used for diagnostic purposes.Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using Real-Time quantitative PCR (RT-qPCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of pneumonia outbreak. This Coronavirus detection kit allows efficient cDNA synthesis and qPCR in a single tube. This probe based one step RTqPCR 2x master mix contains Reverse Transcriptase, Taq DNA polymerase, RNase inhibitor, MgCl2, dNTPs, stabilizers and low ROX reference dye with proprietary buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/ probe sets. This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product Includes:RT-qPCR 2x master mixCDC recommended primer/probe setsCOVID-19 positive control (PTC)
Description: Blood Direct 2x PCR Master Mix enables fast and easy amplification of target DNA from whole blood samples. The master mix is optimized for DNA amplification directly from whole blood samples and does not require prior DNA purification or lysis of the blood sample. The ready-to-use master mix is 2x concentrated and contains all reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Properties:Ready-to-use- Transfer blood sample (1-3 µl) directly into the master mix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform: No complex, time-consuming lysis, DNA isolation and washing stepsHigh sensitivity- Excellent for genes that are frequently examined in the same way in routine screeningSuitable for genomic DNA from blood samples
Description: Blood Direct 2x PCR Master Mix enables fast and easy amplification of target DNA from whole blood samples. The master mix is optimized for DNA amplification directly from whole blood samples and does not require prior DNA purification or lysis of the blood sample. The ready-to-use master mix is 2x concentrated and contains all reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Properties:Ready-to-use- Transfer blood sample (1-3 µl) directly into the master mix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform: No complex, time-consuming lysis, DNA isolation and washing stepsHigh sensitivity- Excellent for genes that are frequently examined in the same way in routine screeningSuitable for genomic DNA from blood samples
Description: Plant direct 2x PCR Mastermixis optimized for the amplification of target DNAdirectly from plant cells or seedsand does not require prior DNA purification. The ready-to-use Mastermix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Plant direct 2x PCR Mastermix is supplied in combinationwith an optimized extraction buffer, which enables rapid lysis (approx. 5-10 min) of your plant parts. Plant direct 2x PCR Mastermix enables quick and easy PCR preparation from plant cells, plant tissue and seeds.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: Tissue Direct 2x PCR master mixis optimized for the amplification of target DNAdirectly from human or animal tissueand does not require prior DNA purification. The ready-to-use master mix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Tissue direct 2x PCR master mix is delivered in combinationwith an optimized extraction buffer, which enables a fast lysis (approx. 5-10 min) of your tissue. Tissue direct 2x PCR master mix enables fast and easy PCR preparation from human and animal tissue as well as cell lysates.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: Tissue Direct 2x PCR master mixis optimized for the amplification of target DNAdirectly from human or animal tissueand does not require prior DNA purification. The ready-to-use master mix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Tissue direct 2x PCR master mix is delivered in combinationwith an optimized extraction buffer, which enables a fast lysis (approx. 5-10 min) of your tissue. Tissue direct 2x PCR master mix enables fast and easy PCR preparation from human and animal tissue as well as cell lysates.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: Plant direct 2x PCR Mastermixis optimized for the amplification of target DNAdirectly from plant cells or seedsand does not require prior DNA purification. The ready-to-use Mastermix is 2-fold concentrated and contains all the reagents required for a polymerase chain reaction (PCR) . The combination of an antibody-blocked hot-start polymerase and an optimized buffer system enables high robustness, sensitivity and reproducibility.Our Plant direct 2x PCR Mastermix is supplied in combinationwith an optimized extraction buffer, which enables rapid lysis (approx. 5-10 min) of your plant parts. Plant direct 2x PCR Mastermix enables quick and easy PCR preparation from plant cells, plant tissue and seeds.Properties:Ready-to-use- transfer lysed sample directly into the Mastermix and start the PCR reactionOne-step- The process takes place in a single reaction vesselQuick & easy to perform- No time-consuming DNA isolationHigh sensitivity- Excellently suited for frequently analyzed genes within routine screening
Description: 5x Hot-Start Taq Multiplex PCR Mix - ready to loadThe 5x Hot-Start Taq Multiplex PCR-Mix is a ready-to-load solution for multiplex and normal hot-start PCRs.The master mix contains all necessary reagents except template DNA, primer and water.The process takes place in a single reaction vessel.The reactions do not have to be pipetted on ice.The dye contained in the mix facilitates pipetting and at the same time serves to visualise the electrophoretic separation.The generated PCR products can be used for TA cloning
This peptide shows activity of antibacterial, antifungal and antiviral significantly to both plants and organisms to the human host. Brugmansia, Capsicum, Datura, Nicotiana, Salpichora, Solanum, Petunia, and Withania is the most studied genera for AMP. Among this genus, Capsicum and Solanum top ranked according to the total number of studies (35% -38% of study) for different AMP.